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To create a reference object, pass the name from a section in the pyreference_config_file.
Remember you can create multiple instances
from pyreference import Reference reference_37 = Reference("GRCh37") reference_38 = Reference("GRCh38") In [9]: reference_37.get_gene_by_name("GATA2").iv Out[9]: <GenomicInterval object '3', [128198264,128212044), strand '-'> In [10]: reference_38.get_gene_by_name("GATA2").iv Out[10]: <GenomicInterval object '3', [128479421,128493201), strand '-'>