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Reduce remaining 23 reptile non-classical FPs on mature-only controls #18

Description

@iskandr

Scope (narrowed 2026-04-15)

Originally filed with 82 FPs (3.8%). Recent work (h-region leaderless gate #42, charged-penalty normalization #43, SP -3 grammar fix #38, motif anchors) has brought total FPs down to 34 (1.6%) on 2155 controls.

The remaining pool is concentrated in one species category:

Category FPs Total Rate
other_vertebrate (mature-only) 23 1011 2.3%
fish 7 379 1.8%
murine 3 75 4.0%
bird 1 305 0.3%
All others 0

The 23 other_vertebrate FPs are almost entirely reptile non-classical class I (E-S / F10 / Q9 gene families from Pogona, Python, Salvator, Anolis, etc.) where mature-only deposits present N-terminal groove helices that mimic SP h-regions.

Approach options (unchanged)

  • Groove-helix fingerprinting: groove helices have different composition than SP h-regions
  • Sequence-length-aware gating: 200-280 aa without Met = likely mature-only
  • N-terminal domain compatibility: well-formed groove from pos 0 = evidence against SP

Success criterion

Drive the other_vertebrate FP rate below 1% (≤10 FPs) without regressing full-length class I SP accuracy on reptile/fish.

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