Hello,
I ran mageck count with --norm-method control to normalize using the control sgRNA library. However, I am confused because in running mageck test subsequently I am getting control sgRNA hits in my gene hit results list. I was not expecting this, because I thought since I was normalizing using the control sgRNA library they would not be appearing in the gene hit list.
Could someone help me understand this better? I also tried asking in the Google groups here but did not receive any answers: https://groups.google.com/g/mageck/c/2mbOMaAfbRw/m/oAvJlKPLAQAJ
Thank you in advance for your help!!
Hello,
I ran mageck count with --norm-method control to normalize using the control sgRNA library. However, I am confused because in running mageck test subsequently I am getting control sgRNA hits in my gene hit results list. I was not expecting this, because I thought since I was normalizing using the control sgRNA library they would not be appearing in the gene hit list.
Could someone help me understand this better? I also tried asking in the Google groups here but did not receive any answers: https://groups.google.com/g/mageck/c/2mbOMaAfbRw/m/oAvJlKPLAQAJ
Thank you in advance for your help!!