whitelist and input barcode lengths are not equal

[Bo-UT]

Hi,

Thanks for developing the workflow!

I am working on 10x genomic multiome (ATAC+Expression) data. The data we got for scATAC-seq have 24bp barcode (16bp barcode + 8 bp spacer). When I started MAESTRO, and I got the error
"whitelist and input barcode lengths are not equal!"

I looked the parameter for scatac-init, and there is no parameter input option for "--barcode-translate" as in chromap. Could you let me know how to deal with this error?

Thanks

Bo

[mourisl]

You may need to manually add --barcode-format option to the chromap command part. What is the 8bp spacer? Do you mean the first 16bp are the real barcode? Thank you.