E-CRISP displays E-score in its output but I find that it is not in the output files of cld. I think this metrics is very important when I want to rank the sgRNAs.
cld does have Doench score and Xu score that can be substitutes but they are not calculated when PAM is not NGG.
Is E-score going to be added to cld in the future version?
http://www.e-crisp.org/E-CRISP/aboutpage.html
The above page describe how to calculate E-score
"Add 1 if the the last 6 bp have a CG content higher then 70 %
Subtract 1 if the entire sequence has GC content > 80 %
Add 1 if sequence is preceded by a G
Add 1 if there are GG in front of the target sequence (opposite the PAM)
Add micro -homology score (is higher when sequence tends to give out of frame deletions)"
It seems quite straight forward except for the "micro-homology score". How to calculate this score?
Thanks a lot in advance.
E-CRISP displays E-score in its output but I find that it is not in the output files of cld. I think this metrics is very important when I want to rank the sgRNAs.
cld does have Doench score and Xu score that can be substitutes but they are not calculated when PAM is not NGG.
Is E-score going to be added to cld in the future version?
http://www.e-crisp.org/E-CRISP/aboutpage.html
The above page describe how to calculate E-score
"Add 1 if the the last 6 bp have a CG content higher then 70 %
Subtract 1 if the entire sequence has GC content > 80 %
Add 1 if sequence is preceded by a G
Add 1 if there are GG in front of the target sequence (opposite the PAM)
Add micro -homology score (is higher when sequence tends to give out of frame deletions)"
It seems quite straight forward except for the "micro-homology score". How to calculate this score?
Thanks a lot in advance.